Amyloid-like Hfq interaction with single-stranded DNA: involvement in recombination and replication in<i>Escherichia coli</i>

Abstract Interactions between proteins and single-stranded DNA (ssDNA) are crucial for many fundamental biological processes, including replication genetic recombination. Thus, understanding detailed mechanisms of these interactions is necessary to uncover regulatory rules occurring in all living cells. The RNA-binding Hfq a pleiotropic bacterial regulator that mediates aspects nucleic acid metabolism. protein notably mRNA stability translation efficiency by using stress-related small RNA as cofactors. In addition, helps compact double-stranded DNA. this paper, we focused on the action ssDNA. A combination experimental methodologies, spectroscopy molecular imaging, has been used probe its amyloid C-terminal region with Our analysis revealed binds Moreover, demonstrate first time drastically changes structure helical parameters ssDNA, mainly due amyloid-like domain. formation nucleoprotein complexes ssDNA unveils important implications